What is so special about ExpressArt technology?
No calibrated amounts are needed.
Whereas all other kits demand for very similar RNA amounts in sample series. ExpressArt avoids the size reductions of other kits (less input RNA gives shorter amplified RNA). Therefore, ExpressArt provides excellent comparability for different sample sizes: RNA from a few cells or from hundreds or thousands of cells obtained from
Laser microdissection, cell sorting, isolated embryos or oocytes.
Amplification of Picogram samples without large primer-derived artefacts.
Combined with the avoided size reductions, this gives excellent results with picograms of input RNA.
Unique ability to use three amplification rounds - without any compromise in data quality and with high concordance in direct comparisons of two and three amplification rounds.
No dead-end with labelled amplified RNAs.
Use Archival Template DNA (#2010-A15), immobilised on magnetic beads, for solid-phase in vitro transcription with unmodified NTPs.
Only if quantitity and quality of the amplified RNA are O.K., simply recover the template DNA and repeat the in vitro transcription reaction with labelled NTP's. Otherwise, use the unmodified RNA to perform an additional amplification round.
No standard RNA quality is needed.
ExpressArt avoids random priming and the patented TRinucleotide priming technology gives highly comparable results in sample series with perfect RNA quality (Agilent RIN>9), reduced quality (RIN 6-9) or low quality (RIN<6).