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GriSP

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GRISP COVIDLately, the GRS Viral DNA/RNA Purification Kit has been successfully used for the detection of SARS-CoV-2, the new coronavirus responsible for the COVID-19 outbreak. The kit provides an efficient and fast method for the purification of high quality viral DNA and RNA from cell-free media (e.g. from serum, plasma, body fluids, and the supernatant from viral infected cell cultures).

 

 

  • GRS VIRAL DNA/RNA PURIFICATION KIT
    • Column based RNA purification from Sputum and viscous nasopharyngeal/oropharyngeal/tracheal aspirates, as well as from swab samples.

  •  XPERT ONE-STEP FAST PROBE
    • Fast and sensitive detection and quantification using RT-qPCR
    • High efficiency in multiplex applications
    • Widely used for SARS-CoV-2 detection worldwide
    • Compatible with WHO and USCDC primers/probes

  • RNAse XTERMINATOR SPRAY
    • Decontamination solution, effective on metal, glass and plastic surfaces.

Xpert Green DNA Stain is a new and safe alternative to ethidium bromide (EtBr) for the visualization of DNA (double-stranded and single-stranded DNA) and RNA in agarose and polyacrylamide gels. The dye has been developed for in-gel staining and is compatible with both UV and Blue LED

 

Properties

Xpert Green DNA Stain has 2 fluorescence excitation wavelengths in the UV range (~270nm; ~290nm) and one in the blue light range (~485nm). Maximum fluorescence emission is at ~525nm (green). Therefore, Xpert Green DNA Stain is compatible with a large variety of gel documentation systems. Yellow/orange or green filters should be used for photography.

Sensitivity

The detection limit of Xpert Green DNA Stain is in the range of 0.5-5.0 ng/band (depending on agarose type and percentage, thickness of the gel, electrophoresis buffer, transilluminator, photo camera quality and settings, etc). Band intensity can be improved by adding Xpert Green DNA Stain to the electrophoresis buffer, especially in case of small DNA fragments that migrate farther. Xpert Green DNA Stain can be used for post-staining, however, with less sensitivity.

 transilluminators.

 

Documents ---->

Xpert directXtract PCR Kit

Sample -> Lysis -> Direct amplification (all in ~1h15 min)

For direct amplification from whole blood, mouse tails, FTA-cards, and FFPE tissue, etc Xpert directXtract PCR kit combines a simple but efficient DNA extraction method with direct amplification using Xpert Fast Hotstart DNA polymerase in a convenient and easy-to-use manner.

This kit can be used with a variety of samples, including whole blood, mouse tails, FTA-cards, and FFPE tissue, and is thus ideal for genotyping and screening, eliminating the need of time-consuming and costly DNA purification methods.

DNA, RNA, and protein, from the same biological sample

 

grisp full workflow

 

 

 

The GRS FullSample Purification Kit provides an efficient and fast method for the simultaneous purification of genomic DNA, total RNA (including miRNA), and total protein from  whole blood and other biological fluids, animal tissues, and cultured cells.

 

 The TripleXtractor directRNA kit combines the strong lysis capability of the monophasic phenol/guanidine thiocyanate solution “TripleXtractor” with a spin column system for convenient and rapid (within 15 min) purification of ultrapure RNA without the need of chloroform phase separation and isopropanol precipitation

 triplextr