Xpert directXtract PCR Kit

Sample -> Lysis -> Direct amplification (all in ~1h15 min)

For direct amplification from whole blood, mouse tails, FTA-cards, and FFPE tissue, etc Xpert directXtract PCR kit combines a simple but efficient DNA extraction method with direct amplification using Xpert Fast Hotstart DNA polymerase in a convenient and easy-to-use manner.

This kit can be used with a variety of samples, including whole blood, mouse tails, FTA-cards, and FFPE tissue, and is thus ideal for genotyping and screening, eliminating the need of time-consuming and costly DNA purification methods.

DNA extraction is carried out in a single tube, without the need of multiple washing steps, therefore minimizing the risk of contamination. DNA is ready for PCR in only 15 minutes! Xpert Fast Hotstart DNA polymerase, with its extension rate of 4-8 kb/min, not only allows for fast PCR, but in combination with optimized buffer ensures high yield and sensitivity. Upon completion of PCR, the reaction is ready for direct loading onto an agarose gel without the need of adding loading buffer.

Product Info & Basic Protocol

Mouse Genotyping App Note 

Performance highlights:
- efficient, fast, and easy, lysis from a variety of samples (15 min)
- fast amplification using Xpert Fast Hotstart Mastermix with dye, for direct loading on gel

Properties:
Amplicon size:  up to 5kb
Extension Rate: 4-8 kb/min
Hotstart:           Yes
A-overhang:      Yes

Applications:  
- Direct PCR
- Mouse Genotyping
- Genetic Screening
- Multiplex PCR

Samples compatible with Xpert directXtract PCR Kit:
> Mouse Tail: 1 to 2 mm (approx 5 mg)
> Mouse Ear Punch: 2 to 4 mm2 (approx 5 mg)
> Animal Tissue: 3 to 30 mg
> FFPE Tissue: 1 mm3 (or 2 mm2 of a 10 μm section)
> Buccal Swab: 1 swab
> Blood (mammalian): 2 to 8μl of fresh blood (with EDTA as anti-coagulant)
> FTA or Guthrie card: 2 mm2
> Hair follicles: 1 to 10 follicles

Components:
Each kit (GE60.0080 for 80rxn; GE60.0480 for 480rxn) consists on Xpert directXtract Lysis Buffer, and Xpert Fast Hotstart mastermix with dye. Each rxn is for 100uL lysis, and for a 25uL PCR reaction.

Storage:
-20ºC for at least 1 year. No loss of performance is detected after 20 freeze/thaw cycles