Mouse Genotyping Workflow
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Published: Friday, 26 July 2019 10:48
GRiSP offers one of the most innovative and high performance options on the market for directPCR applications - Xpert directXtract PCR Kit.
One of the main applications for this kit (despite not limited to) is Mouse Genotyping. Not only it's an application with intensive reagent numbers consumption, but also one where time is highly relevant.
The workflow, show in the flyer on the left, contains three steps, and a total time of only 90 minutes.
It consists on the combination of GE60 (Xpert directXtract PCR Kit) and GB01 (SGTB DNA Electrophoresis Buffer).
Tips for Detecting Phosphoproteins
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Published: Thursday, 11 July 2019 12:07
Protein phosphorylation is very important for protein post-translational modification that controls many cellular processes including metabolism, transcriptional and translation regulation, degradation of proteins, cellular signaling and communication, proliferation, differentiation, and cell survival [1].
Approximately 35% of human proteins are phosphorylated. Phosphoproteins are low in abundance, and, therefore, are, sometimes, challenging to detect and characterize by Western blot and other methods. Western blot is the most common method for detecting and quantifying phosphorylated proteins.
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How to Choose Membrane Protein Isolation Kits
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Published: Thursday, 11 July 2019 09:51
Plasma membrane (PM) proteins play a critical role in a variety of physiological and pathological processes. Signal transduction, molecular transport and cell-cell interactions are all mediated by PM proteins. PM proteins include a variety of important proteins such as neurotransmitter receptors, G-proteins, carriers, voltage-gated ion channels, CD antigens and many drug targets. The detection, characterization, and intracellular trafficking of PM proteins are, therefore, essential for understanding of biological systems.
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