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Protein Separation

 

 

 

 

 

 

 

 

 

 

 

 

 

 


concentrate protein samples and remove buffer components

 

 

 

 

 

Quickly concentrate protein samples and remove buffer components

Afyon SDS-PAGE sample preparation kit is a fast, efficient way to concentrate protein samples and remove buffer components that may interfere with electrophoresis. In less than 10 minutes, samples are ready for SDS-PAGE or immunoblotting. 

 

Features

  • Quickly remove contaminants that can interfere with electrophoresis (GuHCl, urea, Ammonium sulfate, etc)
  • Protein samples ready to load in less than 10 minutes
  • Safe and non-toxic - no DMSO required
  • Many times faster than concentration and buffer exchange via ultrafiltration or dialysis
  • Compatible with SDS-PAGE and downstream Western blotting

Pack your own LC/MS capillary columns

ms-gra- -pressure

What’s the best way to dispense small-volume liquid samples in a controlled manner? Rely on the Next Advance Pressure Injection Cell. Often called a bomb loader, the Pressure Injection Cell is invaluable for two applications:

1. densely packing nanobore capillary columns with solid-phase particles for LC/MS;

2. precisely infusing microliter samples into mass spectrometers without additional transfers, wasted sample or contact with metallic surfaces which adsorb negatively charged molecules.

 

 

Minute™ Protein Extraction Kits

 

 

 

 

The world's leading spin column based protein extraction kits

 

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The Minute™ series of protein extraction kits developed by Invent Biotechnologies, Inc. are excellent tools for rapid and easy protein extraction/isolation from a variety of species including but not limited to mammalian, marine animals, insects, plants and bacteria.

 

Why MinuteTM Kits?

  • Spin column technologies for protein extractionon
  • Super small sample size
  • Cover almost all living organisms

Velum™ precast gels offer perfect protein separation and a novel experience of convenience in handling. Velum™ gels are film-supported and do not need any glass plates for gel electrophoresis and only a small amount of buffer (included with the kit) is required.

Velum™ gels are ideally suited for Red- and near IR-fluorescence protein imaging (T-Red, T-iRed, T-Rex) with a tenfold better signal to noise ratio compared to other gels.

Velum Gel

 

 

 

 

 

 

 

 

velum gel sample

 

 

 

 

 

 

 

 

 

 

 

 

pdf190215_PG_Velum_SAR_eng.pdf

 

 

The most simple blotting method after electrophoresis for 

BEO  blotterVelum™ gels is the contact blotting with the  Beo Dry Blotter

After the electrophoretic procedure the blotting membrane is directly placed on the gel-surface.With the help of dry blotting paper and pressure the proteins are forced to migrate on the membrane.

 Enhanced protein transfer for improved sensitivity

  FLASHBlot Transfer Buffer

Advansta's new FLASHBlot Transfer Buffer allows scientists to achieve increased sensitivity in their Western blots. The buffer provides increased transfer efficiency of all proteins, including large molecular weight proteins that are notoriously difficult to transfer completely. It also increases protein retention on the membrane, so it's easier to detect low-abundance proteins.