Protein Separation

 

 

 

 

 

 

 

 

 

 

 

 

 

 


 

 

 

AdvanBlock™-EIA is a novel blocking solution, optimized to enhance specific antibody-antigen interactions for ELISA. This all-in-one blocking solution and antibody incubation buffer is designed to decrease non-specific binding caused by low quality antibodies and serum matrix effects

 

blocking str

 

 

 

 

 

 

 

 

 

 

 

AdvanBlock-EIA-brochure

 

Minute™ Protein Extraction Kits

 

Yes, it is possible to extract total protein from mammalian cells in just 1 minute!

 

The world's leading spin column based protein extraction kits

 

SPEED EASE OF USE SAMPLE SIZE

 The Minute™ series of protein extraction kits (patents pending) developed by Invent Biotechnologies, Inc. are excellent tools for rapid and easy protein extraction/isolation from a variety of species including but not limited to mammalian, marine animals, insects, plants and bacteria. Unlike any other protein extraction kit in the market, these next generation protein extraction kits employ optimized buffer systems coupled with state of the art proprietary filter cartridge technologies (spin column) for total protein extraction, cell fractionation, and membrane protein isolation. The kits offer numerous advantages over other competing products in terms of speed, ease of use and performance.


Description Cat. No Starting Materials Status of Extracted Proteins Yield
(mg/ml)
Protocol Time
(min)
Total Protein Extraction
(For Animal Cells/Tissues)
SD-001/ SN-002 Cultured Animal Cells and Tissues Denatured/Native 2-8 1-8
Cytoplasmic and Nuclear Fractionation SC-003 Mammalian Cultured Cells/Tissues Native 1-3 6-8
Detergent-Free Total Protein Extraction
(For Animal Cells/Tissues)
SN-006 Cultured Animal Cells and Tissues Denatured/Native 1-4 5-8
Total and Plasma Membrane Protein Isolation SM-005 Mammalian Cultured Cells/Tissues Native Detergent/ EDTA -Free 1-3 20-45
Total and Plasma Membrane Protein Isolation for Plant SM-005-P Plant Cells / Tissues Native Detergent/ EDTA -Free 1-2 <60
Mitochondria Isolation (Mammalian) MP-007 Mammalian Cultured Cells/Tissues Native Detergent/ EDTA-Free 1-3 25-30
Total Protein Extraction
( for Plant Tissues)
SD-008/ SN-009 Fresh Plant Tissues Denatured/Native 2-8 5-8
Detergent-Free Plant Protein Extraction SN-010 Fresh Plant Tissues Native 1-5 6-8
Intact Plant Chloroplast Isolation CP-011 Fresh Plant Tissues (5-200 mg/sample) Chloroplast (>90%) Intact 1-4 5
Bacterial Total Protein Extraction SB-004 Cultured Bacterial Cells Denatured 2-3 2-3
Nuclear Envelope Protein Extraction Kit NE-013 Cultured cells or Cells from Tissues Native 1-3 45
Histone/DNA Binding Protein Extraction Kit HP-014 Cultured cells or Cells from Tissues Denatured 1-2.5 <10
Total Protein Extraction Kit for Microbes with Thick cell walls YT-015 Cultured Microbes Denatured/Native 2-5 <10 min
Detergent-Free Protein Extraction Kit for Microbes with Thick Cell Walls YD-016 Cultured Microbes Denatured/Native 2-4 <10 min
Yeast Mitochondria Enrichment Kit YM-017 Cultured Yeast Native 2-3 1h
Plant Microsomal Membrane Extraction Kit MM-018 Plant tissues Native 2-3 1h
Protein / Nucleic Acid Extraction
(from gel slices)
PN-019 PAGE/Agarose gels Denatured/Native NA 10-20 min
Protein extraction from hair, nail, wool and horn HD-021 Keratinized tissues Denatured 1-2 5 min hand on
Total Protein Extraction from Adipose Tissues AT-022 Adipose Tissues/Adipocytes Native/Denatured 2-3 20 min
Adipose Tissue Fractionation AF-023 Adipose Tissues/Adipocytes Native 0.5-1 20 min
Protein Extraction for Fixed and Embedded Tissues FE-025 FFPE Denatured 2-3 1h

concentrate protein samples and remove buffer components

 

 

 

 

 

Quickly concentrate protein samples and remove buffer components

Afyon SDS-PAGE sample preparation kit is a fast, efficient way to concentrate protein samples and remove buffer components that may interfere with electrophoresis. In less than 10 minutes, samples are ready for SDS-PAGE or immunoblotting. 

 

Features

  • Quickly remove contaminants that can interfere with electrophoresis (GuHCl, urea, Ammonium sulfate, etc)
  • Protein samples ready to load in less than 10 minutes
  • Safe and non-toxic - no DMSO required
  • Many times faster than concentration and buffer exchange via ultrafiltration or dialysis
  • Compatible with SDS-PAGE and downstream Western blotting

The novel ORCA Gel Electrophoresis unit and the corresponding VELUM Precast Gels offer the latest technique in terms of easy handling, resolution and reproducibility for up to 52 protein samples per unit and run.

Up to 4 ORCA Gel Electrophoresis units can be combined (one on top of the other) and run simultaneously.

 

 

 

 

 

 

 

 

ORCA Gel Electrophoresis routine

The ORCA routine is designed for daily protein analysis by SDS-PAGE, SAR-PAGE or IEF. Handling of the system including the correct setting of the corresponding VELUM gels or the electrodes does not require further user education.

 

ORCA Gel Electrophoresis research

The ORCA research offers maximum flexibility for research and development. This system allows for both, 1D gel electrophoresis ans 2D gel elctrophoresis (including Refraction-2D and 2D DIGE).

 

Pack your own LC/MS capillary columns

ms-gra- -pressure

What’s the best way to dispense small-volume liquid samples in a controlled manner? Rely on the Next Advance Pressure Injection Cell. Often called a bomb loader, the Pressure Injection Cell is invaluable for two applications:

1. densely packing nanobore capillary columns with solid-phase particles for LC/MS;

2. precisely infusing microliter samples into mass spectrometers without additional transfers, wasted sample or contact with metallic surfaces which adsorb negatively charged molecules.

 

 

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