HRP substrates

 

 

 

 

Chemiluminescent substrate for ELISA applications

Advansta's substrate ELISABright™ has the highest signal-to-noise ratio of ELISA substrates on the market, making ELISA development a breeze.

 

It has a broad dynamic range for enhanced detection and immediate light generation. ELISABright™ is ideal for enhanced detection of low abundance proteins as well as reduced consumption of costly antibodies.

 

 

 

 

 

ELISABright demonstrates a signal-to-noise ratio 6-times higher than the competition.

 

 

  • Highest signal to noise ratio – for superior performance
  • Broad linear dynamic range – for enhanced detection and precision
  • Immediate light generation – for fast detection
  • Cost effective – and reduced reagent consumption

 

 

Adva Logo

 

 

 

 

 

 

 

Advansta provides products for every step of protein analysis by Western blotting, from electrophoresis to immunodetection.  

WesternBright ECL

The best choice for routine, film-imaged Western blots

 

WesternBright ECL is more sensitive than other chemiluminescent substrates, so you can conduct shorter exposures, load less sample, or use up to ten-times less antibody.  Save time and money with WesternBright ECL. 

  • Stronger signal than Amersham™ ECL or Pierce™ ECL
  • Detect low pg protein amounts
  • Use up to ten-times less antibody

" ... I again later today tried the Quantum solution and it gave about 2 times better results than the one from xxxxx !"

" ..we have now tested the new Advansta substrates extensively and my colleagues are also quite impressed and satisfied! "

 


wb quantum logo

 

 

 

 

 

Quantitative chemiluminescent Western blotting

WesternBright Quantum is formulated to take full advantage of the linear dynamic range of CCD imagers. It provides high sensitivity to detect low abundance proteins, and does not exhibit substrate depletion with high abundance proteins, so has a larger linear dynamic range than other commercially available substrates. A signal linear with respect to protein concentration over 3 orders of magnitude provides the ability to accurately quantify and compare protein amounts in your samples.

 

  • Sensitive – detect attomoles of protein per band.
  • Quantitative – linear range of signal with respect to protein amount exceeds 3 orders of magnitude
  • Low background – for high signal to noise
  • Long lasting signal – image blots hours after substrate incubation
  • Versatile – Optimized for CCD imaging, and compatible with film detection. In addition, chemifluorescent emissions can be detected with fluorescence imaging systems

 

 

 

 

 

 


 wb sirius

See bands you couldn't detect before

 higest sensi

WesternBright Sirius is the most sensitive HRP substrate available from Advansta for chemiluminescent Western blotting. With attomolar sensitivity and a long-lasting signal, WesternBright Sirius allows you to detect bands not visualized with other substrates.

High signal-to-noise and a large dynamic range make it ideal for quantifying low-intensity bands. And, chemifluorescence emission allows WesternBright Sirius to be imaged by fluorescence imagers in addition to traditional CCD imagers and film.