western blot

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  • Advansta Product catalog

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  • Advansta WesternBright

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    Advansta provides products for every step of protein analysis by Western blotting, from electrophoresis to immunodetection.  

    WesternBright ECL

    The best choice for routine, film-imaged Western blots

     

    WesternBright ECL is more sensitive than other chemiluminescent substrates, so you can conduct shorter exposures, load less sample, or use up to ten-times less antibody.  Save time and money with WesternBright ECL. 

    • Stronger signal than Amersham™ ECL or Pierce™ ECL
    • Detect low pg protein amounts
    • Use up to ten-times less antibody

    "... I again later today tried the Quantum solution and it gave about 2 times better results than the one from xxxxx !"

    " ..we have now tested the new Advansta substrates extensively and my colleagues are also quite impressed and satisfied! "

     


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    Quantitative chemiluminescent Western blotting

    WesternBright Quantum is formulated to take full advantage of the linear dynamic range of CCD imagers. It provides high sensitivity to detect low abundance proteins, and does not exhibit substrate depletion with high abundance proteins, so has a larger linear dynamic range than other commercially available substrates. A signal linear with respect to protein concentration over 3 orders of magnitude provides the ability to accurately quantify and compare protein amounts in your samples.

     

    • Sensitive – detect attomoles of protein per band.
    • Quantitative – linear range of signal with respect to protein amount exceeds 3 orders of magnitude
    • Low background – for high signal to noise
    • Long lasting signal – image blots hours after substrate incubation
    • Versatile – Optimized for CCD imaging, and compatible with film detection. In addition, chemifluorescent emissions can be detected with fluorescence imaging systems

     

     

     

     

     

     


     wb sirius

    See bands you couldn't detect before

     higest sensi

    WesternBright Sirius is the most sensitive HRP substrate available from Advansta for chemiluminescent Western blotting. With attomolar sensitivity and a long-lasting signal, WesternBright Sirius allows you to detect bands not visualized with other substrates.

    High signal-to-noise and a large dynamic range make it ideal for quantifying low-intensity bands. And, chemifluorescence emission allows WesternBright Sirius to be imaged by fluorescence imagers in addition to traditional CCD imagers and film.

     

     

     

     

     

     

     

     

     

     


     

     

  • Beo Dry blotter

    Sometimes it may not be the speed that matters but the result...

    The Beo Dry Blotter transfers protein simply based on capillary action with phenomenal results in terms of transfer quality and efficiency. The blotting does neither requires power nor additional buffer. And you can process up 18 blots derived from mini gels or 3 blots from large gels (e.g.Velum™1D, IEF or 2D gels) at the same time.

    BEO  blotter

     Key features

    • Easy to use
    • High quality of protein transfer
    • No additonal buffers, requires no power
    • Takes up to 18 blots from mini gels or 3 large blots from 2D gels

     

     

     

    pdfBeo Dry Blotter product guide.pdf 

  • Blocking solution & antibody-antigen signal enhancer

    electroforesis 

    electroforesis article

  • Blot Development Folders

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    Transparent plastic supports for blot handling and imaging

    Transparent plastic supports for blot handling and imaging

    Advansta's Blot Development Folders are transparent plastic folders that hold your blot while it is exposed to film or a CCD camera.

  • BlotCycler

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  • ChemiPen

    Transform your protein standard into a chemiluminescent marker !

    WesternBright ChemiPen

     

     

     

     

     

     

     

     

    Write or draw on your transfer membranes with the WesternBright ChemiPen.
    With the proprietary "ink" you can make pre-stained protein standards chemiluminescent, annotate your blot with a date or blot number, or check the quality of your HRP substrates.

  • FLASHBlot Transfer Buffer,

     Enhanced protein transfer for improved sensitivity

      FLASHBlot Transfer Buffer

    Advansta's new FLASHBlot Transfer Buffer allows scientists to achieve increased sensitivity in their Western blots. The buffer provides increased transfer efficiency of all proteins, including large molecular weight proteins that are notoriously difficult to transfer completely. It also increases protein retention on the membrane, so it's easier to detect low-abundance proteins.

  • Fusion Advansta

    Customer satisfaction...

    "....as Western blotting with your new camera system is so much more fun than before, we are already running out of ECL solutions again ;)"

    /S

    Fusion-Advansta-

  • FUSION FX

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    pdfSIS_BROCHURE_-_FUSION_FX.pdf

     

     

  • Fusion FX SPECTRA

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  • One additional step...

  • Standardized+Quantitative Western blots = SPL

    learn more...