Application Info

UV-lamp-damenUltraviolet light (UV) forms a part of the invisible radiation which borders on the visible light spectrum. Ultraviolet starts beyond violet, where detection by the human eye ends. Ultraviolet is located at shorter wavelengths compared to visible light radiation and produces high energy potential.

 

 

 

 

The ultraviolet spectrum extends from 180 to 380 nanometers (nm) and is divided into three categories; 

  • Short wave band is also called UV-C. It extends from 180 to 280 nm with an energy peak at 254 nm. The UV-C light has a high bactericidal power and is then appropriate for germicidal applications. Elimination of PCR-contaminations, fluorochemistry, pesticide analysis, mutation studies, mykology, toxikology, mineralogy, polymer curing and control in industry.
  •  Medium wave (UV-B) runs from 280 to 320 nm with an energy peak at 312 nm ideal for DNA/RNA electrophoresis visualization and for mineralogy.

  • Long wave UV light (UV-A) extends from 320 to 380nm with an energy peak at 365 nm. Long wave is also called “black light” or “wood light”. Cultivation of fungi, identification of bacteria, fluorochemistry, pesticide analysis, quality controle, food contamination, archeology and mineralogy.

UV transilluminators are most commonly used in modern laboratories to visualize nucleic acids stained with ethidium bromide or other dyes. There are two different main applications: Analytical gel documentation, i.e. acquisition of images for printing/storage on files Preparative work, i.e. excision of DNA bands from gels, e.g. for cloning purposesThese two applications require transilluminators with different characteristics...

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  application_note_vl0801.pdf

SPL-Appnote-gel-quant

 

Quantitative and reliable protein 1D gel analysis demands a standardized and reproducible system. Proteins of samples separated by SDS- or IEF-PAGE are usually visualized using protein stains. Yet, protein stains are limited in their capabilities.

Due to the low detection sensitivity of Coomassie high amounts of sample are required. Both Coomassie and Silver stains provide a linear range of signals of only two orders of magnitude .

Fluorescent stains provide much better sensitivity and linear range, however, all stains suffer from limitations in terms of reproducibility and require additional time and labor for staining and destaining...